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Mixotrophic growth ofChlorella sorokiniana in outdoor enclosed photobioreactor

February 21st, 2007 | No Comments | Posted in Algae-Cultivation, Algae-Photobioreactor

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Mixotrophic growth ofChlorella sorokiniana in outdoor enclosed photobioreactor

Journal – Journal of Applied Phycology
Publisher – Springer Netherlands
Subject – Biomedical and Life Sciences
Issue –
Volume 8, Number 2 / March, 1996
SpringerLink Date – Tuesday, September 20, 2005

Yuan-Kun Lee1, Sun-Yeun Ding1, Chye-Hock Hoe1 and Chin-Seng Low1
Department of Microbiology, National University of Singapore, Lower Kent Ridge Road, Singapore, 119260

Received: 5 January 1996 Revised: 29 March 1996 Accepted: 2 April 1996


Chlorella sorokiniana was cultured in heterotrophic or mixotrophic mode in outdoor enclosed tubular photobioreactor. The culture temperature was maintained at 32–35 °C. At night, theChlorella culture grew heterotrophically, and 0.1 M glucose was completely consumed. The biomass growth yield of glucose was 0.35 ± 0.001 g-biomass g-glucose–1. During the day, the algal culture grew mixotrophically and the biomass growth yield was 0.49 g-biomass g-glucose–1 in low density culture (initial biomass concentration, Xo = 2 g l–1), 0.56 g-biomass g-glucose–1 in medium density culture (Xo = 4 g l–1) and 0.46 g-biomass g-glucose–1 in high density culture (Xo = 7 g l–1). The daily area productivity of the culture, with Xo = 4 g l–1 corresponded to 127 g-biomass m–2 d–1 during the day and 79 g-biomass m–2 d–1 during the night. In all the cultures, the dissolved O2 concentration increased in the morning, reached the maximum value at noon, and then decreased in the afternoon. The dissolved CO2 concentration remained at 3 mBar in the morning and increased in the afternoon. Glycolate was not found to accumulate in culture medium.

Key words mixotrophic – photosynthetic – Chlorella – outdoor culture – enclosed photoreactor

Original SpringerLink page here

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