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Improved Method for Obtaining Axenic Clones of Planktonic Blue-green Algae

February 21st, 2007 | No Comments | Posted in Algae-Cultivation

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An Improved Method for Obtaining Axenic Clones of Planktonic Blue-green Algae

Journal of Phycology 10 (2), 238–240. doi:10.1111/j.0022-3646.1974.00238.x

Journal of PhycologyVolume 10 Issue 2 Page 238 – June 1974

To cite this article: Wayne W Carmichael, Paul R Gorham (1974)

Wayne W. Carmichael, Department of Botany, University of Alberta, Edmonton, Alberta, Canada

Paul R. Gorham, Department of Botany, University of Alberta, Edmonton, Alberta, Canada


Axenic clones from 5 isolates of Anabaena flosaquae, 1 isolate of Microcystis acruginosa, and 1 isolate of Aphanizomenon flos-aquae were obtained by a combination of steps that provided a 1000-fold reduction in the bacteria-algae ratio and permitted bacteria-free filaments or cells to be isolated and grown from agar pour plates. The first step consisted of the addition of phenol to a dark-treated culture to selectively reduce the numbers of actively growing bacteria while leaving the resting algal cells viable. The next steps involved washing the treated algal suspension on a Millipore filter pad or membrane followed by plating in washed agar containing buffered mineral medium plus vitamins and soil extract. The final steps consisted of incubating the agar pour plates, coring bacteria-free filaments or cells, culturing the agar cores in a buffered mineral medium, and rigorously testing the resulting cultures for bacteriological contamination. Between 50 and 90% of the cores grew, and of these about 50% were judged axenic. The method, with appropriate adaptations, should be suitable for obtaining axenic clones of other freshwater and marine algae.

Original source page here

Nature gave us oil from algae; perhaps we should try Nature’s way again

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algOS – Biodiesel from Algae Open Source

About Oilgae – Oilgae – Oil & Biodiesel from Algae has a focus on biodiesel production from algae while also discussing alternative energy in general. Algae present an exciting possibility as a feedstock for biodiesel, and when you realise that oil was originally formed from algae – among other related plants – you think “Hey! Why not oil again from algae!”

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