; charset=UTF-8" /> Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures | Oilgae Blog

Comprehensive Algae Fuels Report

A detailed report on all aspects of the algae fuel value chain, the Comprehensive Oilgae Report will be of immense help to those who are on the threshold of investing in algae biofuels. More »

Algae-based Wastewater Treatment

Compiled by a diverse team of experts, with experience in scientific and industrial fields, the Comprehensive Report for Wastewater Treatment Using Algae is the first report that provides in-depth analysis and insights on this important field. It uses innumerable data and information from a wide variety of expert sources and market studies, and distills these inputs and data into intelligence and a roadmap that you can use. More »

Comprehensive Guide for Algae-based Carbon Capture

A Comprehensive Guide for Entrepreneurs and Businesses Who Wish to get a Basic Understanding of the Business Opportunities and Industry Dynamics of the Algae-based CO2. More ››

Comprehensive Report on Attractive Algae Product Opportunities

The report provides an overview of the wide range of non-fuel applications of algae – both current and future prospects. It will provide entrepreneurs with an idea of how to derive more benefits from their algal energy ventures. The report provides detailed case studies, success stories and factoids of companies that have been involved in the algae products venture More »

Comprehensive Castor Oil Report

There is no other comprehensive report available for castor oil anywhere in the world. This is the first of its kind, and currently, the only one. More ››

Bioplastics Market & Strategy Advisor

Bioplastics Market & Strategy Advisor, published by the Bioplastics Guide, is a unique guiding framework for businesses and entrepreneurs to chart a way forward provides a critical analysis of the status, opportunities & trends of the global bioplastics sector. More ››

Algae-Useful Substances





Other Novel Applications

Talk to us

Email: consult@eai.in


Call Narsi 7358263274

займ на карту ночью

Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures

You are at: Oilgae Blog. See the complete list of Oilgae Blog articles.

Do you know that oil derived from algae is an exciting renewable fuel possibility? – see Oilgae for more.

Elemental balancing of biomass and medium composition enhances growth capacity in high-density Chlorella vulgaris cultures

Ramkumar K. Mandalam *, Bernhard Palsson

Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109
*Correspondence to Ramkumar K. Mandalam, Department of Chemical Engineering, University of Michigan, Ann Arbor, Michigan 48109

Funded by: NASA; Grant Number: 10-56943

Keywords – Chlorella vulgaris • algae • elemental balancing • medium design • high-density cultures • photoautotrophic growth


The basic requirements for high-density photoautotrophic microalgal cultures in enclosed photobioreactors are a powerful light source and proper distribution of light, efficient gas exchange, and suitable medium composition. This article introduces the concept of balancing the elemental composition of growth medium with biomass composition to obtain high-density cultures. N-8 medium, commonly used for culturing Chlorella vulgaris was evaluated for its capacity to support high-density cultures on the basis of elemental stoichiometric composition of C. vulgaris. This analysis showed that the N-8 medium is deficient in iron, magnesium, sulfur, and nitrogen at high cell densities. N-8 medium was redesigned to contain stoichiometrically balanced quantities of the four deficient elements to support a biomass concentration of 2% (v/v). The redesigned medium, called M-8 medium, resulted in up to three- to fivefold increase in total chlorophyll content per volume of culture as compared to N-8 medium. Further experiments showed that addition of each of the four elements separately to N-8 medium did not improve culture performance and that balanced supplementation of all four deficient elements was required to yield the improved performance. Long-term (24 d) C. vulgaris culture in M-8 medium showed continuous increase in chlorophyll content and biomass throughout the period of cultivation. In contrast, the increase in chlorophyll content and biomass ceased after 7 and 12 d, respectively in N-8 medium, demonstrating the higher capacity of M-8 medium to produce biomass. Thus, the performance of high cell density photobioreactors can be significantly enhanced by proper medium design. The elemental composition of the biomass generated is an appropriate basis for medium design.

Original John Wiley link here

Nature gave us oil from algae; perhaps we should try Nature’s way again

Oilgae – Oil & Biodiesel from Algae
Oilgae Blog; The complete list of Oilgae Blog articles.
algOS – Biodiesel from Algae Open Source

About Oilgae – Oilgae – Oil & Biodiesel from Algae has a focus on biodiesel production from algae while also discussing alternative energy in general. Algae present an exciting possibility as a feedstock for biodiesel, and when you realise that oil was originally formed from algae – among other related plants – you think “Hey! Why not oil again from algae!”

Leave a Reply 4914 views, 1 so far today | | posted by

Leave a Reply

You must be logged in to post a comment.

Hot Algae Products (Click to know more)
Omega 3 Spirulina Astaxanthin Chlorella

Get in Touch

Send us a message to know more on how oilgae.com can help your organization.