Genetic techniques for oilgae 8Genetic techniques like transgenesis or gene silencing are of great importance to produce algal strains of desired characteristics.
Transgenesis means introduction of a foreign gene into the genome of algae to improve its oil producing efficiency, resistance, or stress hardiness etc. It is done by semi-disrupting the cell wall through agitating with glass beads, electroporation etc. and then infecting with artificially engineered virus with desired gene plus a selection marker gene so that they are incorporated in the genome of algae.
To separate algae that have acquired this gene from others where the process has failed we use the selection marker i.e. antibiotic resistance is seen only in algae where transgenesis has succeded, or such algae are identified by fluorescent or biochemical msrkers. This process is known as genetic transformation.
Wed August 04 2010 10:36:57 AM by Aayush 663 viewsLogin to Post a Comment