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Genetic techniques for oilgae 8

Genetic techniques like transgenesis or gene silencing are of great importance to produce algal strains of desired characteristics.
Transgenesis means introduction of a foreign gene into the genome of algae to improve its oil producing efficiency, resistance, or stress hardiness etc. It is done by semi-disrupting the cell wall through agitating with glass beads, electroporation etc. and then infecting with artificially engineered virus with desired gene plus a selection marker gene so that they are incorporated in the genome of algae.
To separate algae that have acquired this gene from others where the process has failed we use the selection marker i.e. antibiotic resistance is seen only in algae where transgenesis has succeded, or such algae are identified by fluorescent or biochemical msrkers. This process is known as genetic transformation.
Wed August 04 2010 05:06:57 AM by Aayush 1797 views

Comments - 7

  • Manohar wrote:
    Wed August 04 2010 11:03:39 PM

    Which companies are using these techniques/
    Which universitites are using these ?
    What is the progress so far ?

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  • Abomohra wrote:
    Thu August 05 2010 03:15:25 PM

    Thanks Aayush for this post, But do you know more details about transformation in eukaryotic algae?? Do you have papers for that??

    Thanking you in advance.
    Best Regards,
    Abomohra

    Vote Up! 4 Vote Down! 0

  • Manohar wrote:
    Thu August 05 2010 10:42:35 PM

    Prototroph

    He is the other guy interested in genetics and in
    eukaryotic algae. @ abomohra you may want to befriend him and talk to him.

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  • Pami wrote:
    Fri August 06 2010 06:14:54 AM

    Can you be specific why selection procedures failed...I mean anbiotic resistance like kanamysin resistance or bioluminance should succed!!!....Are u saying that the algae on a whole general tolerant to antibiotics making slectio process impossible!!

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  • Power1921 wrote:
    Fri August 06 2010 10:20:49 AM

    transformation in algae is a new horizon that still needs to be explored..

    Vote Up! 0 Vote Down! 0

  • Manohar wrote:
    Sat August 07 2010 12:27:50 AM

    Can u guys explain what Craig Venter is doing for Exxon Mobile !

    Vote Up! 0 Vote Down! 0

  • Sun October 31 2010 04:20:57 PM

    I am sure that transformation of microalgae is not a dream ,let us do it from ABC 

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