Genetic techniques for oilgae 8
Genetic techniques like transgenesis or gene silencing are of great importance to produce algal strains of desired characteristics.Transgenesis means introduction of a foreign gene into the genome of algae to improve its oil producing efficiency, resistance, or stress hardiness etc. It is done by semi-disrupting the cell wall through agitating with glass beads, electroporation etc. and then infecting with artificially engineered virus with desired gene plus a selection marker gene so that they are incorporated in the genome of algae.
To separate algae that have acquired this gene from others where the process has failed we use the selection marker i.e. antibiotic resistance is seen only in algae where transgenesis has succeded, or such algae are identified by fluorescent or biochemical msrkers. This process is known as genetic transformation.
Wed August 04 2010 10:36:57 AM by Aayush
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Which companies are using these techniques/
Which universitites are using these ?
What is the progress so far ?
Thanks Aayush for this post, But do you know more details about transformation in eukaryotic algae?? Do you have papers for that??
Thanking you in advance.
Best Regards,
Abomohra
Prototroph
He is the other guy interested in genetics and in
eukaryotic algae. @ abomohra you may want to befriend him and talk to him.
Can you be specific why selection procedures failed...I mean anbiotic resistance like kanamysin resistance or bioluminance should succed!!!....Are u saying that the algae on a whole general tolerant to antibiotics making slectio process impossible!!
transformation in algae is a new horizon that still needs to be explored..
Can u guys explain what Craig Venter is doing for Exxon Mobile !
I am sure that transformation of microalgae is not a dream ,let us do it from ABC