Rajshree

City - New Delhi

Country - India

About - RAJSHREE E Mail: rajshree70@gmail.com 328 ? RZH, Rajnagar -2 Street no.-9, Palam colony, New Delhi INDIA OBJECTIVE: I seek a position of Bio-tech specialist in one of the company where I can use my past experience as well as my academic qualification for the benefit of the company. I am quite confident that my creative ideas as well as theoretical knowledge will help me to take the company to great heights. WORK EXPERIENCE: (Total 2 months) 5th June 2008 ? 5th August2008: Designation: Trainee Hospital: Army research and referral Hospital, New Delhi Work field: Worked in the pathology labs all departments, (Immunology, Biochemistry, Macro biology, Hematology, and Microbiology). EDUCATION (Throughout 1st Class) Post Graduation: Currently Pursuing -Master of Technology (Biotechnology) University School of Biotechnology, Guru Gobind Singh Indraprastha University, Delhi Graduation: Bachelor of Technology (Biotechnology) (2006-2010) University School of Biotechnology, Guru Gobind Singh Indraprastha University, Delhi Higher Secondary: XIIth (2006) Air Force senior secondary school, Palam Delhi cantonment, New Delhi High School: Xth (2003) Air Force senior secondary school, Palam Delhi cantonment, New Delhi RESEARCH EXPERIENCE: (A) B.tech biotechnology final year project in the field of Immunology ?Turmeric and aloe vera extracts on human PBMCs? and was graded Excellent. The objectives of the project were to saw the Immunomodulatory action of turmeric and Aloe Vera extract on in-vitro cultured peripheral blood mononuclear cells by estimating protein content of these cells when incubated at different conditions. The study involved 1. preparation of turmeric rhizome extract 2. preparation of aloe vera extract 3. DMEM-G culture media preparation 4. Isolation of PBMCs 5. Cell number counting and cell viability check 6. In-vitro culture of PBMCs in different conditions of plant extracts and with their neat solvents 7. Cell lysis of PBMCs after 24 hours of incubation in different conditions 8. Protein content analysis from adherent and non-adherent cells by Bradford protein analysis method TECHNIQUES KNOWN: ADVANCES IN AGRICULTURAL TECHNIQIUES: Molecular breeding MOLECULAR BIOLOGY TECHNIQUES: DNA sequencing, gene isolation & expression studies, gene libraries PLANT TISSUE CULTURE TECHNIQUES: Isolation of single cells, suspension cultures, culture of single cells, germplasm Storage ANIMAL TISSUE CULTURE TECHNIQUES: Culturing, sub culturing, karyotyping, Cryopreservation IMMUNOLOGICAL TECHNIQUES: ELISA, Immuno Electrophoresis, Antigen capture BASIC TECHNIQUES: Ion exchange chromatography, affinity chromatography, gel chromatography, UV spectroscopy, visible spectroscopy, Polymerase Chain reaction (PCR)

Role - Student